Quantification of DNA Adducts by Using Liquid Chromatography/Tandem Mass Spectrometry
نویسندگان
چکیده
منابع مشابه
Quantification of Protein Phosphorylation by Liquid Chromatography−Mass Spectrometry
The identification and quantification of specific phosphorylation sites within a protein by mass spectrometry has proved challenging when measured from peptides after protein digestion because each peptide has a unique ionization efficiency that alters with modification, such as phosphorylation, and because phosphorylation can alter cleavage by trypsin, shifting peptide distribution. In additio...
متن کاملLiquid Chromatography Tandem Mass/Mass Spectrometry for the Quantification of Fudosteine in Human Serum without Precolumn Derivatization
A quantitative analysis method for fudosteine in human serum by high-performance liquid chromatography-electrospray ionization tandem mass spectrometry (HPLC-ESI/MS/MS) was established, which shows high sensitivity and selectivity. The mobile phase composition was 75% 20 mM acetic acid and 25% acetonitril, which was pumped at a flow rate of 0.40 mL/min. The overall chromatographic run time was ...
متن کاملLiquid Chromatography Tandem Mass/Mass Spectrometry for the Quantification of Fudosteine in Human Serum without Precolumn Derivatization
A quantitative analysis method for fudosteine in human serum by high-performance liquid chromatography-electrospray ionization tandem mass spectrometry (HPLC-ESI/MS/MS) was established, which shows high sensitivity and selectivity. The mobile phase composition was 75% 20 mM acetic acid and 25% acetonitril, which was pumped at a flow rate of 0.40 mL/min. The overall chromatographic run time was ...
متن کاملPlasma Free Metanephrines Quantitation with Automated Online Sample Preparation and Liquid ChromatographyTandem Mass Spectrometry
Recently, we reported an LC-MS/MS method for measuring Pmets using ion-pairing solid phase extraction (IP-SPE) and porous graphitic carbon (PGC) column chromatography1, 2. Although the method is fast and analytically sensitive, it can be further improved by automating the offline sample preparation with online sample preparation technology, which is more timeand cost-effective. Thermo Scientifi...
متن کاملQuantification of sirolimus by liquid chromatography-tandem mass spectrometry using on-line solid-phase extraction.
Quantification of the new immunosuppressant sirolimus (syn. rapamycin; Rapamune) in whole blood by chromatography is essential for its clinical use since no immunoassay is available although monitoring is mandatory. Here we report on a rapid and convenient liquid chromatography (LC-tandem mass spectrometry method and describe our practical experience with its routine use. Whole blood samples we...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of the Mass Spectrometry Society of Japan
سال: 2009
ISSN: 1340-8097,1880-4225
DOI: 10.5702/massspec.57.301